| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SNRK recombinant protein (Position: D48-E750) was used as the immunogen for the SNRK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNRK Antibody / SNF-related serine/threonine-protein kinase is a anti-SNRK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SNRK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
SNRK antibody identifies a kinase containing an N-terminal catalytic domain with the conserved activation loop threonine residue, followed by a unique C-terminal regulatory region that mediates protein-protein interactions. Activation of SNRK occurs via phosphorylation by upstream kinases such as LKB1. Once activated, SNRK phosphorylates downstream targets that regulate glucose uptake, fatty acid oxidation, and mitochondrial function. It plays a key role in maintaining cellular energy homeostasis under metabolic stress.
In cardiac tissue, SNRK supports myocardial energy efficiency and contractile function by modulating lipid metabolism and mitochondrial biogenesis. Loss of SNRK impairs mitochondrial respiration and increases susceptibility to metabolic disorders. In neurons, SNRK contributes to synaptic vesicle trafficking and axonal growth through phosphorylation of cytoskeletal and vesicle-associated proteins. Additionally, SNRK modulates inflammatory pathways by phosphorylating NF-kappaB regulators and promoting macrophage polarization toward an anti-inflammatory phenotype.
Pathway involvement of SNRK includes the AMPK signaling network, where it acts downstream of metabolic stress sensors to control autophagy, oxidative metabolism, and lipid catabolism. The kinase also participates in developmental signaling related to angiogenesis and cardiogenesis. Studies have shown that SNRK expression increases during fasting or hypoxia, supporting its role in adaptive metabolic regulation. Structurally, SNRK shares homology with other AMPK family kinases such as MARK2 and NUAK1, containing conserved ATP-binding and activation loop motifs.
Clinically, alterations in SNRK expression have been associated with obesity, insulin resistance, and heart disease. In the nervous system, SNRK dysregulation may contribute to neuroinflammation and neurodegeneration. Genome-wide association studies have linked SNRK variants to lipid metabolism disorders and coronary artery disease risk. The kinase's regulatory control of energy metabolism and inflammatory responses makes it a promising therapeutic target for metabolic and cardiovascular disease research.
Immunohistochemical staining using SNRK antibody shows cytoplasmic and nuclear localization in cardiac myocytes, neurons, and hepatocytes. The SNRK antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
