| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SNTA1 recombinant protein (Position: Q76-H480) was used as the immunogen for the SNTA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNTA1 Antibody / Alpha-1-Syntrophin is a anti-SNTA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SNTA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, Alpha-1-syntrophin is a 505-amino-acid cytoplasmic protein of approximately 55 kilodaltons containing multiple modular domains, including a pleckstrin homology (PH) domain, a PDZ domain, and a C-terminal syntrophin unique (SU) region. These domains mediate interactions with membrane proteins such as neuronal nitric oxide synthase (nNOS), ion channels, and cytoskeletal elements. The protein localizes to the sarcolemma in muscle cells and to synaptic and submembranous regions in neurons.
The SNTA1 antibody is widely used in muscle biology, neuroscience, and cardiovascular research to study membrane organization, signal transduction, and cytoskeletal dynamics. Western blot analysis detects a 55 kilodalton band corresponding to Alpha-1-syntrophin, while immunofluorescence reveals membrane-associated and cytoplasmic staining patterns consistent with its adaptor function. This antibody provides a reliable tool for examining the molecular architecture of membrane-associated signaling complexes.
Functionally, SNTA1 regulates nitric oxide signaling, calcium homeostasis, and ion channel activity by linking nNOS and other signaling proteins to the DAPC. Mutations in SNTA1 have been associated with long QT syndrome and cardiac arrhythmias, suggesting a role in maintaining proper electrical signaling in cardiomyocytes. Alpha-1-syntrophin also influences cellular migration and polarization by anchoring signaling complexes at specific membrane domains. The SNTA1 antibody supports research into cytoskeletal organization, membrane signaling, and neuromuscular disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
