| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SNX16 recombinant protein (Position: I84-D344) was used as the immunogen for the SNX16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNX16 Antibody / Sorting nexin-16 is a anti-SNX16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: SNX16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
SNX16 is encoded by the SNX16 gene located on human chromosome 8p21.3. The protein is approximately 62 kilodaltons and localizes primarily to early endosomes. It participates in the formation of tubular endosomal structures that mediate receptor sorting between degradation and recycling pathways. SNX16 functions independently of the retromer complex and instead promotes membrane tubulation through its coiled-coil domain. It regulates trafficking of receptors such as EGFR and TrkB, influencing growth factor signaling and neuronal development.
The SNX16 antibody typically identifies a 40-50 kilodalton protein by western blot and demonstrates punctate cytoplasmic staining characteristic of endosomal localization. Functional studies show that depletion of SNX16 disrupts endosomal tubulation, alters cargo recycling, and can impair signaling pathways dependent on receptor localization. Overexpression of SNX16 enhances endosomal membrane curvature and promotes receptor clustering, demonstrating its mechanochemical role in shaping endosomes.
In neurons, SNX16 contributes to dendritic spine maintenance and receptor turnover, impacting synaptic plasticity. It may also participate in autophagy regulation through endosome-lysosome fusion. Aberrant SNX16 expression has been linked to neurodegenerative disorders and cancer, where altered trafficking pathways contribute to signaling dysregulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
