| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SNX25 recombinant protein (Position: H48-K584) was used as the immunogen for the SNX25 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SNX25 Antibody / Sorting nexin 25 is a anti-SNX25 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SNX25
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
SNX25 is encoded by the SNX25 gene located on human chromosome 4q24. The protein is approximately 580 amino acids long and contains a conserved phox homology (PX) domain that binds phosphatidylinositol-3-phosphate and a Bin/Amphiphysin/Rvs (BAR) domain that senses and induces membrane curvature. These structural domains enable SNX25 to participate in tubular endosome formation and cargo retrieval to the Golgi or plasma membrane.
The SNX25 antibody detects a 67 kilodalton band by western blot and shows cytoplasmic and vesicular staining by immunofluorescence. SNX25 interacts with transforming growth factor beta receptor complexes and promotes their endocytic degradation, acting as a negative regulator of transforming growth factor beta signaling. By controlling receptor turnover, SNX25 influences cellular proliferation, differentiation, and immune tolerance.
SNX25 also regulates Toll-like receptor trafficking in macrophages, affecting cytokine production and innate immune responses. In neurons, SNX25 participates in the endosomal sorting of synaptic receptors and contributes to synaptic plasticity. Its expression is dynamically regulated by inflammatory cues and cellular stress, linking endosomal remodeling to signaling adaptation.
Aberrant expression of SNX25 has been associated with cancer progression and immune dysregulation. Downregulation enhances transforming growth factor beta activity, promoting fibrosis and epithelial-mesenchymal transition, while overexpression suppresses pro-inflammatory signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
