| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SOAT1 recombinant protein (Position: R41-Y548) was used as the immunogen for the SOAT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SOAT1 Antibody / Sterol O-acyltransferase 1 is a anti-SOAT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: SOAT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, SOAT1 antibody identifies a 550-amino-acid multi-pass membrane enzyme that catalyzes the formation of cholesterol esters by transferring fatty acyl groups from acyl-CoA to the hydroxyl group of cholesterol. These neutral esters are stored in cytoplasmic lipid droplets, buffering free cholesterol concentrations within membranes. SOAT1 activity is critical for maintaining cellular lipid balance and protecting against cholesterol-induced cytotoxicity.
The SOAT1 gene is located on chromosome 1q25.2 and encodes a protein embedded in the endoplasmic reticulum membrane. It operates as a key metabolic switch regulating cholesterol storage and membrane composition. In macrophages, SOAT1 converts excess cholesterol into cholesteryl esters, preventing foam cell formation and atherogenesis. In steroidogenic tissues, it contributes to the availability of cholesterol precursors for hormone synthesis. Dysregulation of SOAT1 disrupts lipid homeostasis and is implicated in metabolic disorders, neurodegeneration, and cardiovascular disease.
SOAT1 functions alongside SOAT2, which is primarily expressed in the liver and intestine, while SOAT1 is ubiquitously expressed in peripheral tissues. Its activity is regulated by intracellular cholesterol levels, oxysterols, and acyl-CoA availability. Pharmacological inhibition of SOAT1 has been explored as a strategy to reduce atherosclerotic plaque formation and neurotoxic cholesterol accumulation in Alzheimer's disease.
SOAT1 antibody is widely used in lipid metabolism, cardiovascular, and neurobiology research. It is suitable for immunoblotting, immunofluorescence, and enzyme localization studies to evaluate intracellular cholesterol esterification. This antibody enables the investigation of lipid droplet formation, cholesterol trafficking, and sterol regulatory pathways. In metabolic studies, SOAT1 serves as a marker of intracellular cholesterol storage and lipid imbalance.
Structurally, SOAT1 is an endoplasmic reticulum membrane protein containing multiple transmembrane domains and conserved histidine residues essential for catalytic function. The enzyme operates as a homotetramer, with each subunit contributing to the catalytic center.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
