| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SON recombinant protein (Position: M1-Y2426) was used as the immunogen for the SON antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SON Antibody / Protein SON is a anti-SON Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear speckles.
Key elements and design rationale
- Target: SON
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Functionally, SON antibody identifies a 2,426-amino-acid nuclear protein characterized by multiple low-complexity regions, a G-patch domain, and RNA recognition motifs. SON interacts with splicing factors, including SRSF2, PRPF8, and U2AF65, promoting efficient intron removal and exon definition. It acts as a scaffold within nuclear speckles, organizing splicing regulators and transcription machinery. SON also participates in the transcriptional regulation of genes governing cell cycle progression, differentiation, and chromatin stability.
The SON gene is located on chromosome 21q22.11 and is highly conserved across vertebrates. It is ubiquitously expressed but enriched in proliferating cells, reflecting its role in transcription-coupled RNA processing. Depletion of SON results in global splicing defects, preferentially affecting long genes with weak splice sites and GC-rich regions. These defects lead to downregulation of critical regulators of DNA replication, chromosome segregation, and pluripotency.
Mutations or haploinsufficiency of SON cause ZTTK syndrome (Zhu-Tokita-Takenouchi-Kim syndrome), a developmental disorder characterized by intellectual disability, growth retardation, and brain malformations. SON also influences genome organization by tethering active chromatin domains near nuclear speckles, thereby enhancing transcriptional efficiency. In cancer, overexpression of SON supports rapid proliferation and survival by stabilizing mRNAs of oncogenic pathways, including MYC and WNT signaling components.
SON antibody is widely used in molecular biology and nuclear organization research. It is suitable for immunoblotting, immunofluorescence, and RNA immunoprecipitation to examine SON's role in splicing and chromatin architecture. SON serves as a nuclear speckle marker in microscopy, distinguishing transcriptionally active nuclear domains. In developmental biology, SON detection aids in characterizing splicing fidelity and gene expression networks critical for tissue differentiation.
Structurally, SON contains numerous serine/arginine-rich and glycine-rich motifs that mediate protein and RNA interactions. Phosphorylation regulates its association with spliceosomal complexes and nuclear bodies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
