| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SOS1 recombinant protein (Position: H177-A1288) was used as the immunogen for the SOS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SOS1 Antibody / Son of sevenless 1 is a anti-SOS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: SOS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SOS1 is encoded by the SOS1 gene on human chromosome 2p22.1. The protein is approximately 1333 amino acids long and consists of several domains: an N-terminal histone-like fold, a Dbl homology (DH) domain, a pleckstrin homology (PH) domain, and a C-terminal catalytic region responsible for Ras activation. SOS1 is recruited to the plasma membrane through interaction with adaptor proteins such as GRB2 following growth factor stimulation.
The SOS1 antibody detects a 155 kilodalton protein in western blot assays and shows cytoplasmic and membrane localization by immunofluorescence. Upon receptor activation, SOS1 facilitates Ras activation at the plasma membrane, leading to phosphorylation of ERK and other downstream targets. Through its DH-PH domains, SOS1 also activates Rac, linking actin cytoskeleton remodeling to growth factor signaling.
SOS1 plays essential roles in development, cell proliferation, and differentiation. Germline mutations in SOS1 cause Noonan syndrome, a developmental disorder characterized by short stature and congenital heart defects. In cancer, overexpression or dysregulation of SOS1 contributes to aberrant Ras activation and uncontrolled growth signaling. Its activity is finely tuned by feedback loops involving phosphorylation, ubiquitination, and membrane recruitment dynamics.
Because of its central position in the Ras signaling cascade, SOS1 is a valuable target for understanding receptor-driven proliferation and oncogenic transformation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
