| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SOX9 recombinant protein (Position: M1-Q496) was used as the immunogen for the SOX9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SOX9 Antibody / SRY-box transcription factor 9 is a anti-SOX9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SOX9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, SOX9 antibody identifies a 509-amino-acid nuclear protein that binds to promoter and enhancer regions of cartilage-specific genes such as COL2A1 and ACAN. SOX9 forms dimers through its HMG domain and recruits transcriptional coactivators to drive cartilage matrix production and skeletal formation. It also plays roles in sex differentiation by activating AMH (anti-M�llerian hormone) transcription during gonadal development.
The SOX9 gene is located on chromosome 17q24.3 and is expressed in developing cartilage, gonads, and neural crest-derived tissues. SOX9 is a master regulator of chondrocyte differentiation and is essential for maintaining progenitor cell identity in multiple lineages.
Pathologically, mutations or haploinsufficiency of SOX9 cause campomelic dysplasia, a severe skeletal malformation syndrome with sex reversal. Aberrant SOX9 expression also contributes to cancer progression and tissue fibrosis. Research using SOX9 antibody supports studies in developmental biology, transcription regulation, and regenerative medicine.
SOX9 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors involved in chondrogenesis and lineage specification.
Structurally, SRY-box transcription factor 9 contains an HMG DNA-binding domain and transactivation regions that mediate chromatin remodeling and transcriptional activation. This antibody supports detailed analysis of SOX9's regulatory mechanisms in development and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
