SP100 Antibody

Overview

The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.

This anti-SP100 antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone PCRP-SP100-1B9, Mouse IgG2b, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.

Key elements and design rationale

• Target: SP100
• Format: Purified
• Localization: Nucleus
• Species reactivity: Human
• Applications (listed): ELISA, WB, IF, IP, FACS, IHC-P
• Conjugate: Unconjugated
• Clone and antibody class: Monoclonal (mouse origin), clone PCRP-SP100-1B9, Mouse IgG2b

Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.

Biological background

SP100 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.

Research relevance and current trends

• Profiling SP100 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
• Combining antibody-based detection with multi-omics or imaging readouts to link SP100 signal with phenotype.
• Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.

Common research applications

• ELISA
• WB
• IF
• IP
• FACS
• IHC-P

Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.

Notes for experimental interpretation

• Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
• Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
• Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.