| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SP110 recombinant protein (Position: M1-E615) was used as the immunogen for the SP110 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SP110 Antibody / SP110 nuclear body protein is a anti-SP110 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SP110
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, SP110 antibody identifies a 688-amino-acid protein that acts as a coactivator for nuclear hormone receptors and NF-kappaB-dependent transcription. SP110 regulates gene expression in macrophages and lymphocytes, enhancing interferon-stimulated gene transcription and pathogen defense. It also contributes to chromatin remodeling and transcriptional repression of inflammatory genes through PML-associated nuclear bodies.
The SP110 gene is located on chromosome 2q37.1 and is highly expressed in hematopoietic tissues. SP110 expression is induced by interferon signaling and pathogen exposure, positioning it as a regulator of innate and adaptive immune responses. Its nuclear localization and coactivator activity support immune system homeostasis and pathogen resistance.
Pathologically, SP110 gene mutations are associated with hepatic veno-occlusive disease with immunodeficiency (VODI), a rare autosomal recessive disorder causing liver failure and impaired immunity. Abnormal expression of SP110 has also been linked to autoimmune conditions and susceptibility to intracellular infections such as tuberculosis. Research with SP110 antibody supports studies in nuclear organization, immune regulation, and transcriptional control.
SP110 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to detect nuclear body components and transcriptional cofactors.
Structurally, SP110 contains N-terminal SAND and coiled-coil domains that mediate DNA and protein binding, as well as nuclear localization signals that target it to PML bodies. This antibody enables analysis of SP110's role in transcriptional activation and immune regulation under interferon signaling conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
