| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPA17 recombinant protein (Position: M1-K136) was used as the immunogen for the SPA17 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPA17 Antibody / Sperm autoantigenic protein 17 is a anti-SPA17 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi), cell memebrane.
Key elements and design rationale
- Target: SPA17
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, IHC, WB
Biological background
SPA17 was initially identified as an autoantigen in patients with infertility and autoimmune disorders, reflecting its immunogenic nature. It is also classified as a cancer-testis antigen due to its restricted normal expression in testicular tissue and aberrant expression in various malignancies, including ovarian, prostate, and lung cancers. This re-expression in tumors has made SPA17 a target of interest for cancer immunotherapy and tumor biomarker research. Functionally, SPA17 promotes sperm-egg interaction by binding to carbohydrate residues on the zona pellucida and may participate in intracellular signaling during acrosomal exocytosis.
The SPA17 gene is located on chromosome 11q24.2 and encodes a 151-amino acid protein that forms homodimers. The protein associates with the outer acrosomal membrane and flagellar structures, supporting motility and fertilization capacity. Beyond reproduction, SPA17 has been detected in immune and neural tissues, where it may modulate cAMP-mediated signaling pathways. Studies have shown SPA17 interaction with protein kinase A (PKA) regulatory subunits, linking it to cellular adhesion and signal transduction.
Immunohistochemical analysis using SPA17 antibody reveals strong staining in testis, epididymis, and certain tumor tissues. The antibody is widely used to investigate sperm biology, fertility disorders, and tumor immunogenicity. SPA17 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
