| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPAG16 recombinant protein (Position: D28-F619) was used as the immunogen for the SPAG16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPAG16 Antibody / Sperm-associated antigen 16 is a anti-SPAG16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SPAG16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
SPAG16 is encoded by the SPAG16 gene on human chromosome 2q34. Two main isoforms are produced: SPAG16L (long form), expressed predominantly in the testis and required for sperm motility, and SPAG16S (short form), expressed in ciliated tissues such as the trachea and ependyma. The long isoform localizes to the axonemal central pair microtubule complex, while the short isoform resides in the nucleus where it regulates gene transcription during spermatogenesis. Both isoforms are evolutionarily conserved, reflecting a fundamental role in ciliary function across species.
Research employing the SPAG16 antibody has identified the protein as critical for maintaining the integrity of the central apparatus in motile cilia. Knockout or mutation of Spag16 in mouse models results in male infertility, impaired ciliary beating, and hydrocephalus due to defective cerebrospinal fluid flow. Western blot analysis typically detects bands ranging from 70�95 kDa, depending on isoform and tissue type. Immunofluorescence reveals localization along flagella and cilia, consistent with its axonemal function.
SPAG16 interacts with other axonemal proteins such as SPAG6, SPAG17, and components of the radial spoke complex, forming an essential molecular network for rhythmic ciliary movement. Its nuclear isoform (SPAG16S) regulates expression of genes required for sperm maturation and differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
