| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPATA6 recombinant protein (Position: R58-F488) was used as the immunogen for the SPATA6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPATA6 Antibody / Spermatogenesis-associated protein 6 is a anti-SPATA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi apparatus, Vesicles.
Key elements and design rationale
- Target: SPATA6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Spermatogenesis-associated protein 6 contributes to the structural integrity of the connecting piece between the nucleus and flagellum, ensuring proper transmission of motility forces. The SPATA6 antibody supports visualization of this protein during spermiogenesis, revealing dynamic redistribution patterns correlated with microtubule organization. Loss of SPATA6 disrupts flagellar biogenesis and causes male infertility in model organisms, emphasizing its essential role in germ-cell architecture.
Beyond testicular expression, Spermatogenesis-associated protein 6 is detected in ciliated epithelial cells, suggesting broader participation in cilium assembly and anchoring. The SPATA6 antibody facilitates these studies and aids in understanding conserved mechanisms that govern axoneme formation and basal-body docking. Its structural role links it to cytoskeletal regulators and motor proteins such as dyneins and kinesins.
Mutations in the SPATA6 gene have been associated with asthenozoospermia, characterized by reduced sperm motility. The SPATA6 antibody enables quantification of protein expression in sperm samples and cell lines, providing valuable diagnostic and mechanistic insights. Additionally, SPATA6 participates in the assembly of intercellular junctions during germ-cell development, influencing Sertoli�spermatid adhesion and polarity.
The SPATA6 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, typically showing localization to sperm tail and manchette regions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
