| Field | Specification |
|---|---|
| Target | |
| Alternative names | SPTBN1; SPTB2; Fodrin beta chain; Beta-II spectrin; Spectrin, non-erythroid beta chain 1 |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide, corresponding to amino acids 650-700 of Human Spectrin β II. |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Spectrin is an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin α I and spectrin β I are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β II (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Defects in the SPTB gene encoding spectrin β I are the cause of spherocytosis type I (SPH1), a disorder characterized by severe hemolytic anemia. Another disorder due to defects of SPTB is called elliptocytosis or elliptocytosis 3 (EL3). The human SPTB gene maps to chromosome 14q22-q23.2 and encodes the 2,137 amino acid, 246 kDa erythroid form of β-spectrin.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Spectrin β II. Spectrin β II (M687) polyclonal antibody detects endogenous levels of Spectrin β II protein.
Validated Applications
| Applications | Immunohistochemistry |
|---|---|
| IHC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide, corresponding to amino acids 650-700 of Human Spectrin β II.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide, corresponding to amino acids 650-700 of Human Spectrin β II. |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 275 kDa |
| Swiss-Prot | Q01082 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Spectrin β II research from Bioworld Technology Inc, or contact us for alternative formats.
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