| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPIDR recombinant protein (Position: E273-E904) was used as the immunogen for the SPIDR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPIDR Antibody / Scaffold protein involved in DNA repair is a anti-SPIDR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus.
Key elements and design rationale
- Target: SPIDR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, ICC, WB, FACS
Biological background
SPIDR contains multiple interaction domains, including coiled-coil regions and binding motifs that mediate association with RAD51 paralogs and BRCA2. Following DNA damage, SPIDR localizes to nuclear foci corresponding to double-strand break sites, where it promotes RAD51 filament formation and repair fidelity. By stabilizing repair intermediates, SPIDR ensures proper resolution of recombination events and maintenance of genomic stability. Depletion of SPIDR impairs DNA repair efficiency and increases chromosomal aberrations, underscoring its importance in genome maintenance.
The SPIDR antibody is used in DNA repair, cancer, and cell cycle research to analyze homologous recombination pathways and genomic stability. Western blot analysis identifies a 90 kilodalton band corresponding to SPIDR, while immunofluorescence shows punctate nuclear foci following genotoxic stress. This antibody enables researchers to track DNA repair factor recruitment and assess homologous recombination efficiency.
Defects in SPIDR expression or localization are associated with hypersensitivity to DNA-damaging agents and increased genomic instability, contributing to cancer susceptibility. SPIDR also cooperates with checkpoint proteins such as ATR and CHK1 to coordinate DNA repair with replication stress responses. The SPIDR antibody supports these investigations by providing specific detection in western blotting, flow cytometry, and immunofluorescence applications.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
