| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SPINK4 was used as the immunogen for the SPINK4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPINK4 Antibody / Serine peptidase inhibitor Kazal-type 4 is a anti-SPINK4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC) with listed reactivity in Human. Reported localization: Golgi, Nucleus.
Key elements and design rationale
- Target: SPINK4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC
Biological background
Functionally, SPINK4 antibody identifies a 86-amino-acid protein predominantly expressed in goblet cells of the small and large intestine. SPINK4 binds and inhibits trypsin-like proteases, preventing excessive proteolysis in the mucosal environment. It contributes to epithelial integrity and immune defense by maintaining the protective mucus barrier.
The SPINK4 gene is located on chromosome 9p13.3 and is expressed specifically in intestinal and colonic mucosa. Its expression is upregulated during inflammation and epithelial injury, reflecting its protective and regulatory role in the gut.
Pathologically, elevated SPINK4 levels have been reported in colorectal cancer, inflammatory bowel disease, and gastrointestinal infections. It is a potential biomarker for epithelial stress and differentiation states in intestinal disorders. Research using SPINK4 antibody supports studies in mucosal immunology, epithelial biology, and cancer biomarker discovery.
SPINK4 antibody is validated for western blotting, ELISA, and immunohistochemistry to detect secreted protease inhibitors.
Structurally, Serine peptidase inhibitor Kazal-type 4 consists of a single Kazal inhibitory domain stabilized by disulfide bonds, which confers high affinity for target proteases. This antibody enables investigation of SPINK4's function in mucosal protection and intestinal homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
