| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPOCK1 recombinant protein (Position: H35-E427) was used as the immunogen for the SPOCK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPOCK1 Antibody / Testican 1 is a anti-SPOCK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SPOCK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, SPOCK1 antibody identifies a 438-amino-acid protein characterized by domains for proteoglycan attachment and calcium binding. SPOCK1 inhibits metalloproteinases such as MMP2 and cathepsin L, thereby influencing extracellular matrix turnover. It also interacts with glycosaminoglycans and cell surface receptors to regulate adhesion, proliferation, and differentiation. In the nervous system, SPOCK1 supports axon guidance, synaptic stability, and neurogenesis. In non-neural tissues, it contributes to angiogenesis and wound repair.
The SPOCK1 gene is located on chromosome 5q31.2 and is expressed in brain, kidney, heart, and various epithelial tissues. Expression is regulated during embryogenesis and tissue regeneration and is modulated by growth factors including TGFbeta and EGF. SPOCK1's matrix-associated properties make it a key component in maintaining extracellular homeostasis.
Pathologically, elevated SPOCK1 expression has been linked to tumor progression and metastasis in cancers such as lung, liver, and prostate carcinoma. By modulating MMP activity and promoting epithelial-mesenchymal transition (EMT), SPOCK1 enhances invasion and angiogenesis. Conversely, in neural tissues, dysregulation affects axonal pathfinding and synapse formation. Research using SPOCK1 antibody supports studies in cancer metastasis, extracellular matrix biology, and neural development.
SPOCK1 antibody can be validated for western blotting, immunohistochemistry, and ELISA to detect extracellular matrix proteins.
Structurally, Testican-1 contains an N-terminal proteoglycan domain with glycosaminoglycan attachment sites, followed by Kazal-type protease inhibitor and calcium-binding domains. Its modular organization allows inhibition of proteolytic enzymes and stabilization of extracellular matrix components. This antibody enables analysis of SPOCK1's function in matrix remodeling, neural morphogenesis, and tumor biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
