| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPON1 recombinant protein (Position: E247-H611) was used as the immunogen for the SPON1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPON1 Antibody / Spondin 1 is a anti-SPON1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SPON1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, ELISA
Biological background
SPON1 is encoded by the SPON1 gene located on human chromosome 11p15.2. The protein is approximately 91 kilodaltons and consists of a signal peptide, reelin domain, thrombospondin type I repeats, and a C-terminal region that mediates cell surface binding. Spondin-1 is secreted into the extracellular matrix where it interacts with integrins and heparan sulfate proteoglycans to promote neurite extension and cell attachment.
A SPON1 antibody can detect a 90-95 kilodalton band by western blot and reveals extracellular and pericellular staining in immunohistochemistry. During embryonic development, Spondin-1 guides commissural axons across the midline and contributes to axonal fasciculation. In adult tissues, it participates in vascular remodeling and ECM integrity. Spondin-1 is also implicated in Alzheimer's disease pathology, where altered expression affects amyloid precursor protein processing.
Beyond neural function, Spondin-1 contributes to chondrogenesis, osteogenesis, and tumor angiogenesis. Its expression is upregulated during wound repair and inflammation, suggesting a role in tissue regeneration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
