| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPOPL recombinant protein (Position: H173-Q361) was used as the immunogen for the SPOPL antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPOPL Antibody / Speckle-type POZ protein-like is a anti-SPOPL Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear.
Key elements and design rationale
- Target: SPOPL
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Structurally, SPOPL is a 367-amino-acid nuclear protein of approximately 41 kilodaltons containing a BTB/POZ domain responsible for CUL3 interaction and a MATH domain that recognizes substrate proteins. Unlike its paralog SPOP, which forms higher-order oligomers, SPOPL exists as a stable dimer, influencing substrate specificity and ubiquitination efficiency. SPOPL localizes to nuclear speckles and chromatin-associated regions, where it coordinates the degradation of transcriptional co-regulators and chromatin modifiers.
The SPOPL antibody is widely used in cell biology, molecular genetics, and oncology research to study ubiquitin signaling, transcriptional repression, and nuclear protein quality control. Western blot analysis detects a 41 kilodalton band corresponding to SPOPL, while immunofluorescence shows punctate nuclear staining consistent with speckle localization. This antibody enables exploration of nuclear ubiquitin pathways and their effects on gene expression and chromatin remodeling.
Functionally, SPOPL forms part of the CUL3-RBX1 ubiquitin ligase complex, targeting proteins involved in chromatin regulation and transcriptional activation. By controlling the degradation of co-regulators, SPOPL influences gene silencing, cell cycle progression, and developmental signaling. Dysregulation of SPOPL expression has been linked to cancer, where altered ubiquitin signaling contributes to genomic instability and transcriptional imbalance. The SPOPL antibody provides a reliable reagent for studying these nuclear pathways and understanding adaptor-mediated substrate selection in ubiquitin ligase systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
