| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SPSB2 recombinant protein (Position: Q17-Q263) was used as the immunogen for the SPSB2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPSB2 Antibody / SplA/Ryanodine receptor domain and SOCS box containing protein 2 is a anti-SPSB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SPSB2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, SPSB2 antibody identifies a 293-amino-acid protein that regulates signaling through inducible nitric oxide synthase (iNOS), erythropoietin, and other cytokine-related pathways. SPSB2 binds iNOS, targeting it for proteasomal degradation, thereby controlling nitric oxide production and inflammatory responses. Through this mechanism, SPSB2 contributes to feedback inhibition in immune signaling and maintains cellular redox balance.
The SPSB2 gene is located on chromosome 12q24.31 and encodes a member of the SPSB family, which includes SPSB1, SPSB3, and SPSB4. Each family member functions as an adaptor in ubiquitin ligase complexes, characterized by their conserved SPRY and SOCS domains. SPSB2 expression is high in macrophages, spleen, and liver, reflecting its role in immune regulation and metabolic processes.
Pathologically, dysregulated SPSB2 activity affects nitric oxide signaling, contributing to inflammatory disorders, cancer progression, and metabolic stress. By promoting degradation of iNOS, SPSB2 helps terminate inflammatory responses and prevent tissue damage. Reduced SPSB2 levels may prolong nitric oxide signaling, leading to oxidative stress or chronic inflammation.
SPSB2 antibody is suitable for western blotting, immunohistochemistry, and immunoprecipitation to study protein degradation mechanisms, immune signaling, and nitric oxide regulation.
Structurally, SPSB2 contains an N-terminal SPRY domain mediating substrate recognition and a C-terminal SOCS box that binds elongin B/C, Cullin 5, and Rbx2 to assemble an E3 ligase complex. This modular configuration enables selective targeting of regulatory proteins for degradation, linking SPSB2 to diverse cellular pathways governing inflammation and metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
