| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Spt6/SUPT6H recombinant protein (Position: A564-Q1639) was used as the immunogen for the SPT6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SPT6 Antibody / SUPT6H / Transcription elongation factor SPT6 is a anti-SPT6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SPT6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, SPT6 is a large nuclear protein (~170 kDa) containing tandem SH2 domains at the C-terminus that mediate direct binding to the phosphorylated C-terminal domain of RNA Pol II. The protein also includes acidic and basic regions that interact with nucleosomes, histones H3/H4, and elongation factors. SPT6 belongs to the transcription elongation factor family conserved across eukaryotes and forms complexes with other regulators such as IWS1, PAF1C, and FACT to coordinate chromatin structure with RNA synthesis.
Functionally, SPT6 promotes nucleosome reassembly behind the elongating RNA polymerase and prevents cryptic transcription initiation within coding regions. It also regulates histone modifications, such as H3K36 methylation, by recruiting SETD2 and other histone modifiers during elongation. In addition, SPT6 influences mRNA splicing and 3'-end processing by stabilizing Pol II on gene bodies. Through its dual role as a histone chaperone and elongation factor, SPT6 ensures faithful transcriptional output and epigenetic stability.
Loss or dysregulation of SPT6 disrupts transcription elongation and chromatin organization, leading to genomic instability and aberrant gene expression. Mutations have been associated with developmental defects, cancer, and neurodegenerative disorders. SPT6 also participates in transcription-coupled DNA repair and chromatin restoration after stress. Pathway associations include RNA polymerase II transcription, chromatin remodeling, and epigenetic regulation. During development, SPT6 expression supports neuronal differentiation and transcriptional fidelity in proliferating cells.
The SPT6 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
