| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SQRDL/SQOR recombinant protein (Position: R12-E321) was used as the immunogen for the SQOR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SQOR Antibody / Sulfide quinone oxidoreductase / SQRDL is a anti-SQOR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: SQOR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, SQOR antibody identifies a 450-amino-acid mitochondrial inner membrane enzyme containing a covalently bound FAD cofactor. SQOR transfers electrons from hydrogen sulfide to ubiquinone, producing reduced ubiquinol for the respiratory chain while forming a persulfide on its catalytic cysteine residue. This reaction detoxifies sulfide and integrates it into mitochondrial energy metabolism.
The SQOR gene is located on chromosome 15q26.1 and is widely expressed, with highest levels in liver, kidney, and heart. It is localized to the mitochondrial inner membrane facing the intermembrane space, forming part of the mitochondrial sulfide oxidation pathway along with ETHE1, TST, and SUOX. SQOR activity ensures sulfide homeostasis and prevents respiratory inhibition by excess H2S.
Pathologically, SQOR deficiency disrupts sulfide metabolism, leading to mitochondrial dysfunction and oxidative stress. Altered expression has been observed in cardiovascular disease, ischemia-reperfusion injury, and metabolic disorders. By maintaining sulfide balance, SQOR contributes to redox regulation and cellular protection. Research using SQOR antibody aids in studying sulfur metabolism, mitochondrial bioenergetics, and redox biology.
SQOR antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect mitochondrial sulfur oxidation enzymes.
Structurally, Sulfide:quinone oxidoreductase features an FAD-binding domain and transmembrane regions anchoring it to the inner mitochondrial membrane. Its catalytic mechanism involves cysteine persulfide intermediates and electron transfer to coenzyme Q. This antibody enables investigation of SQOR's enzymatic role in sulfur and redox metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
