| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SRA1 recombinant protein (Position: M1-S224) was used as the immunogen for the SRA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SRA1 Antibody / Steroid receptor RNA activator 1 is a anti-SRA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: SRA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, SRA1 antibody identifies a 236-amino-acid nuclear protein that binds steroid hormone receptors such as estrogen receptor (ESR1), androgen receptor (AR), and glucocorticoid receptor (NR3C1). SRA1 enhances receptor-dependent transcription by stabilizing coactivator complexes containing p160 family members (NCOA1-3) and histone acetyltransferases such as CBP/p300. The RNA form of SRA1 acts as a scaffold that promotes chromatin remodeling and transcriptional activation.
The SRA1 gene is located on chromosome 5q31.3 and is broadly expressed in hormone-responsive tissues including breast, uterus, prostate, and skeletal muscle. Its expression is induced by steroid hormones, forming a positive feedback loop that amplifies nuclear receptor signaling. SRA1 plays key roles in reproductive physiology, energy metabolism, and cellular differentiation.
Pathologically, aberrant SRA1 expression contributes to hormone-dependent cancers and metabolic syndromes. Overexpression enhances estrogen and androgen signaling, driving tumor growth in breast and prostate cancer. Mutations or reduced expression can impair receptor-mediated gene regulation and contribute to infertility or metabolic dysfunction. Research using SRA1 antibody supports studies in transcriptional regulation, nuclear receptor biology, and hormone signaling.
SRA1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcriptional coactivators.
Structurally, Steroid receptor RNA activator 1 protein (SRAP) contains RNA recognition and protein interaction domains that allow dual regulatory capacity through RNA-protein networks. Its modular architecture supports cooperative interactions with multiple transcriptional cofactors. This antibody enables detailed analysis of SRA1's function in steroid hormone receptor signaling and coactivation mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
