| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SRARP recombinant protein (Position: K30-S147) was used as the immunogen for the SRARP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SRARP Antibody / Steroid receptor-associated and regulated protein is a anti-SRARP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: SRARP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
SRARP is expressed in hormone-responsive tissues including the brain, ovary, testis, and endometrium. It interacts with nuclear receptors such as estrogen receptor alpha (ERalpha) and retinoic acid receptor (RAR), modulating transcriptional output in a ligand-dependent manner. Through these interactions, SRARP influences gene networks governing cell cycle progression, neurogenesis, and reproductive tissue differentiation. Its expression is hormonally regulated, increasing in response to estrogen and decreasing under conditions of hormone withdrawal.
The SRARP antibody is widely used in endocrinology, neurobiology, and developmental research to study transcriptional regulation and steroid receptor signaling. Western blot analysis typically identifies a 27 kilodalton band corresponding to SRARP, while immunofluorescence shows nuclear localization in hormone-responsive cells. This antibody enables characterization of SRARP's role in receptor signaling and target gene transcription.
Dysregulation of SRARP expression has been linked to altered reproductive function and hormone-dependent cancers, including breast and ovarian carcinoma. SRARP may function as a modulator balancing proliferative and differentiation signals in response to hormonal cues. The SRARP antibody provides a key reagent for exploring transcriptional networks influenced by steroid receptors.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
