| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SRBD1 recombinant protein (Position: M613-K955) was used as the immunogen for the SRBD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SRBD1 Antibody / S1 RNA-binding domain-containing protein 1 is a anti-SRBD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Mitochondria.
Key elements and design rationale
- Target: SRBD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, SRBD1 antibody identifies a 1,364-amino-acid protein that interacts with ribosomal proteins and RNA helicases to facilitate ribosome maturation and rRNA processing. SRBD1 binds pre-rRNA intermediates and assists in their folding, modification, and assembly into mature ribosomal subunits. It also participates in RNA quality control and nonsense-mediated decay pathways, ensuring translational fidelity and RNA stability. In addition to its RNA-binding activity, SRBD1 influences transcriptional regulation and DNA replication by interacting with chromatin-associated complexes.
The SRBD1 gene is located on chromosome 2q31.1 and is expressed in proliferative tissues such as bone marrow, liver, and testis. Expression increases during active cell growth and decreases under stress or differentiation signals. SRBD1 activity coordinates ribosome assembly with cellular growth requirements, making it essential for protein synthesis homeostasis.
Pathologically, SRBD1 mutations and altered expression have been linked to cancer, developmental disorders, and glaucoma susceptibility. Overexpression may promote tumorigenesis by enhancing ribosome biogenesis and proliferation, while reduced SRBD1 disrupts translation and leads to genomic instability. Research using SRBD1 antibody supports studies in RNA biology, ribosome assembly, and oncogenic transformation.
SRBD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect RNA-binding proteins.
Structurally, S1 RNA-binding domain-containing protein 1 possesses an S1-type RNA-binding motif and multiple coiled-coil and low-complexity regions that mediate protein-protein and RNA interactions. This modular structure allows SRBD1 to serve as a scaffold linking RNA metabolism to nuclear and cytoplasmic processes. This antibody enables the investigation of SRBD1's role in ribosome formation and its regulation of gene expression and cellular growth.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
