| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
Serum response factor (SRF) is a transcription factor that controls genes governing cytoskeletal organization, cell proliferation, and immediate-early gene expression. SRF activity is regulated by two principal input branches: the MAPK-ELK pathway and the RhoA-actin-MRTF pathway. Signaling through G12/G13-coupled receptors activates RhoGEFs such as p115-RhoGEF, which stimulate the small GTPase RhoA. RhoA-driven actin polymerization releases myocardin-related transcription factors to co-activate SRF, inducing genes involved in cytoskeletal remodeling, proliferation, and metastatic progression. An SRF-RE reporter built on serum response elements from the α-actin promoter selectively captures this RhoA-dependent branch of GPCR signaling.
Product Description & Applications
The SRF-RE Reporter Lentivirus is a transcription-factor reporter system designed to detect G12α/G13α-mediated activation of RhoA signaling through serum response factor (SRF). It contains tandem repeats of consensus SRF response elements derived from the human α-actin promoter, optimized to read out RhoA-dependent transcriptional activation while minimizing the broad serum responsiveness of general SRE reporters. The construct couples these elements to a minimal TATA-box promoter and an upstream enhancer to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase), with a constitutively expressed selection marker for stable polyclonal cell line generation. It is used to study GPCR-RhoA-SRF signaling, with readout by microscopy, flow cytometry, or luminometry. Supplied as purified lentiviral particles suitable for primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the SRF Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
