| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SRPX recombinant protein (Position: E40-T464) was used as the immunogen for the SRPX antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SRPX Antibody / Sushi repeat-containing protein X-linked is a anti-SRPX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SRPX
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
SRPX antibody identifies a multifunctional protein expressed in various tissues, including the brain, lung, and vascular endothelium. Within the extracellular environment, SRPX acts as an adhesion molecule that facilitates interactions between cells and matrix components. It is upregulated during wound healing and tissue remodeling, suggesting a role in extracellular matrix turnover and endothelial migration. SRPX contains three sushi domains, an N-terminal signal peptide, and glycosylation sites that support secretion and stability in the extracellular milieu.
In the nervous system, SRPX contributes to synapse formation and refinement. A related paralog, SRPX2, has been implicated in synaptic development and language-associated brain disorders, suggesting possible functional overlap within this protein family. SRPX also interacts with urokinase-type plasminogen activator receptor (uPAR) and complement components, supporting its role in both immune regulation and neurodevelopment. The presence of sushi domains links SRPX to complement control proteins that regulate immune activation and inflammation. Functional studies suggest that SRPX can influence cell migration, angiogenic responses, and matrix protease activity, making it relevant in vascular biology and tumor progression.
Clinically, altered SRPX expression has been observed in cancer, where it may contribute to tumor angiogenesis and metastasis. Overexpression has been reported in certain carcinomas, whereas decreased expression may impair extracellular signaling and cell adhesion. SRPX mutations have been studied in relation to X-linked intellectual disability and speech delay, although functional evidence remains limited. The SRPX gene resides in a genomic region frequently associated with inherited neurological disorders and may have regulatory overlap with neighboring genes involved in synaptic signaling.
From a structural standpoint, SRPX shares sequence homology with complement control proteins and contains conserved cysteine residues essential for disulfide bond formation. Its extracellular localization allows it to function as a scaffold in tissue remodeling and immune recognition. The protein family classification places SRPX among sushi domain-containing adhesion molecules, emphasizing its dual role in immune defense and neuronal plasticity.
Immunohistochemical analysis using SRPX antibody demonstrates extracellular and membrane-associated localization in endothelial cells, neurons, and epithelial tissues. The SRPX antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
