| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SSH1 recombinant protein (Position: E489-Y921) was used as the immunogen for the SSH1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SSH1 Antibody / Slingshot protein phosphatase 1 is a anti-SSH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cytoskeleton, nucleus.
Key elements and design rationale
- Target: SSH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, SSH1 antibody identifies a 1,047-amino-acid phosphatase that activates cofilin by removing inhibitory phosphate groups at serine 3. SSH1 operates downstream of Rho family GTPases and is controlled by 14-3-3 proteins, oxidative signals, and phosphorylation. It promotes lamellipodia formation and cytoskeletal reorganization during migration and neuronal outgrowth.
The SSH1 gene is located on chromosome 12q24.13 and is expressed in brain, muscle, and epithelial tissues. SSH1 integrates signals from LIM kinase and Rac/Cdc42 pathways to coordinate actin turnover and filament severing. Through cofilin activation, SSH1 drives rapid actin recycling necessary for directional movement and cell shape maintenance.
Pathologically, altered SSH1 activity contributes to cancer metastasis, neurological disorders, and cardiac hypertrophy. Dysregulated actin remodeling via SSH1-cofilin imbalance leads to defective migration and cytoskeletal instability. Research using SSH1 antibody supports studies in cytoskeletal dynamics, phosphatase signaling, and cell motility.
SSH1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect actin-regulating phosphatases.
Structurally, Slingshot protein phosphatase 1 contains an N-terminal cofilin-binding region and a catalytic domain related to the dual-specificity phosphatase family. This antibody aids in analyzing SSH1's control over actin filament turnover and cellular movement.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
