| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SSH3 recombinant protein (Position: Q108-Q620) was used as the immunogen for the SSH3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SSH3 Antibody / Slingshot homolog 3 is a anti-SSH3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear speckles, cytoplasm, plasma membrane.
Key elements and design rationale
- Target: SSH3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, SSH3 antibody identifies a 575-amino-acid cytoplasmic enzyme that acts downstream of Rho family GTPases. SSH3 dephosphorylates cofilin at serine 3, restoring its actin-severing activity and promoting filament turnover. The protein also interacts with scaffolding components such as 14-3-3 proteins and LIM kinases, coordinating actin remodeling in response to extracellular cues.
The SSH3 gene is located on chromosome 3p21.31 and is widely expressed in brain, muscle, and epithelial tissues. SSH3 contributes to cell shape changes, migration, and polarity through dynamic actin regulation. In neurons, it participates in synaptic plasticity and axon guidance by fine-tuning cytoskeletal rearrangements during development and signaling.
Pathologically, aberrant SSH3 activity disrupts cytoskeletal organization and has been associated with cancer metastasis and neurological disorders. Overactivation enhances cell motility and invasion, whereas loss of function impairs normal morphogenesis. Research with SSH3 antibody supports studies on actin regulation, neuronal development, and signal transduction.
SSH3 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry, enabling precise detection of SSH3 in various cell types.
Structurally, SSH3 contains an N-terminal catalytic domain related to the dual-specificity phosphatase family and C-terminal regions mediating protein-protein interactions. Its localization and activity are regulated by phosphorylation and 14-3-3 binding. This antibody facilitates analysis of SSH3's role in actin filament turnover and cell motility control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
