Stable Receptor Related Protein (RAP) LRP1 Inhibitor

What it means: Target is the identity of the recombinant protein you are purchasing. The target on this page is RAP.

How to interpret here: Use RAP as your “match checkpoint” before ordering—confirm it aligns with your assay goal (antigen for antibody validation, standard for quantitation, binding partner for kinetics, or functional reagent). Conjugate/tag status is no tag; plan capture/detection and tag controls accordingly. Purity is >90% as determined by SDS-PAGE.—important for low-background binding assays and standards. Molecular weight guidance 42.00 kDa helps you QC by SDS‑PAGE/Western. Physical form is Lyophilized; plan reconstitution/aliquoting based on this. Storage is Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.—use it to prevent degradation before your run. Reconstitution guidance is Reconstitute in sterile water for a stock solution.—follow it to preserve activity/solubility. Choose this when target + origin + construct + handling match your workflow; consider an alternative if you need full‑length vs fragment, a different origin, a different tag, or native PTMs.

Why it matters for buying:

• Biological relevance: Your results depend on whether the protein matches the pathway/epitope/interaction you intend to test.
• Reagent compatibility: Antibodies and ligands can be highly sensitive to construct boundaries, PTMs, and tags.
• Reproducibility: Clear target/construct details reduce “it binds in paper X but not in my hands” surprises.

Practical guidance:

• Confirm the binding site: If you know the epitope or functional domain, verify it overlaps the expressed region before ordering for a critical experiment.
• Plan a pilot: Test a small aliquot first to confirm binding/behavior in your buffer, surface chemistry, and detection method.
• Use controls: Include buffer-only and an unrelated protein control; add a tag-control when a conjugate/tag is present.
• Cell-based awareness: Endotoxin is listed as Please contact with the lab for this information.—important for immune readouts and stimulation assays.
• Buffer compatibility: Formulation is Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.; confirm it won’t interfere with coating, conjugation, or coupling chemistries.

What to watch out for:

• PTM-dependent epitopes: Proteins expressed in bacteria often lack mammalian glycosylation—choose a host compatible with your epitope/function.
• Tag interference: Tags can create extra epitopes or steric effects; interpret binding with tag controls when needed.
• Construct mismatch: A fragment can be perfect for epitope mapping but may not behave like full‑length protein in functional assays.

What it means: Conjugate describes whether the protein includes a tag or labeling chemistry that changes capture/detection and sometimes solubility. The value shown here is no tag.

How to interpret here: Treat no tag as your capture/detection choice—select it based on how you plan to immobilize the protein, detect it, and control background. This listing is for RAP. Purity >90% as determined by SDS-PAGE. influences background; tag capture can sometimes reduce non‑specific adsorption versus passive coating. No Tag means you should plan tag‑free immobilization (passive coating or chemical coupling) and avoid anti‑tag detection strategies.

Why it matters for buying:

• Workflow fit: Tags can dramatically simplify capture and improve immobilization reproducibility.
• Interpretation: Tags introduce additional epitopes and can change apparent binding kinetics; controls are essential.
• Downstream compatibility: Some downstream chemistries (coupling, labeling) are sensitive to buffer components and tags.

Practical guidance:

• Use tag controls: Include an unrelated protein with the same tag to identify tag-driven background.
• Optimize surface density: Overloading capture surfaces can cause steric crowding and reduce binding.
• Confirm tag location: If your binding site is near a terminus, tag placement can matter—check construct notes.

What to watch out for:

• Tag interference: Tags can mask epitopes or introduce non‑specific binding; compare to tag‑free constructs if needed.
• Assuming cleavability: Do not assume tags are removable unless a protease site/protocol is provided.
• Surface artifacts: Tag capture can change orientation vs passive coating—interpret kinetics accordingly.

What it means: Form describes the physical state of the supplied protein (e.g., lyophilized powder vs liquid solution). The value shown here is Lyophilized.

How to interpret here: Use Lyophilized to plan how you will prepare the protein for your assay—reconstitution steps, concentration accuracy, aliquoting, and stability after thawing. Lyophilized proteins are shipped dry for stability, but your results depend on careful reconstitution (gentle mixing, correct buffer, and complete dissolution). Reconstitution guidance: Reconstitute in sterile water for a stock solution.. Formulation/buffer: Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. (check compatibility with labeling/coupling and assay buffers). Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. (aliquot and minimize freeze–thaw). For RAP, a pilot dilution series after preparation helps confirm expected behavior.

Why it matters for buying:

• Stability and shipping risk: Lyophilized proteins often tolerate shipping better; liquids can be more temperature-sensitive.
• Concentration accuracy: Incomplete dissolution or pipetting viscous solutions can distort concentrations and assay signals.
• Downstream compatibility: Buffers/stabilizers in liquid formats can interfere with coating, conjugation, or coupling chemistries.

Practical guidance:

• Aliquot immediately: After reconstitution/thaw, aliquot into single-use portions to avoid repeated freeze–thaw.
• Mix gently: Avoid vigorous vortexing for sensitive proteins; gentle inversion or slow pipetting reduces aggregation risk.
• Verify solubility: If you see particulates, allow time to dissolve and consider centrifugation if compatible with your assay.
• Let it fully dissolve: After adding buffer, wait and mix gently until the solution is clear; concentration is unreliable if undissolved material remains.

What to watch out for:

• Freeze–thaw damage: Multiple cycles can reduce binding/activity and increase aggregation.
• Adsorption losses: Low-concentration proteins can stick to tubes; use low-bind plastics when possible.
• Buffer mismatch: Reconstituting in the wrong buffer can precipitate the protein or alter binding—follow recommended guidance.