| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STAM2 recombinant protein (Position: K134-L525) was used as the immunogen for the STAM2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STAM2 Antibody / Signal transducing adaptor molecule 2 is a anti-STAM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: STAM2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, ICC, WB, FACS
Biological background
Functionally, STAM2 antibody identifies a 512-amino-acid cytosolic protein containing VHS, SH3, and ubiquitin-interacting motifs. STAM2 binds to HGS, clathrin, and ubiquitinated receptors, guiding cargo sorting into multivesicular bodies. By directing receptor trafficking, STAM2 attenuates signaling from growth factor receptors, cytokine receptors, and immune complexes.
The STAM2 gene is located on chromosome 2p13.1 and is expressed ubiquitously, with high abundance in immune and epithelial cells. STAM2 functions downstream of activated receptors such as EGFR, ensuring signal termination through endosomal sorting. Its interaction with tyrosine kinases and deubiquitinating enzymes provides regulatory balance between receptor activation and degradation.
Pathologically, disrupted STAM2 expression or mutation can impair receptor turnover, leading to prolonged signaling and oncogenic transformation. Overactivation of endocytic pathways involving STAM2 has been implicated in cancer and immune dysregulation. Research using STAM2 antibody helps elucidate receptor trafficking, ubiquitin signaling, and endosomal dynamics.
STAM2 antibody is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect ESCRT-0 complex components and study receptor endocytosis.
Structurally, STAM2 contains an N-terminal VHS domain for cargo recognition, a central SH3 domain mediating protein interactions, and C-terminal ubiquitin-binding motifs. This configuration allows STAM2 to integrate receptor internalization with signaling termination. This antibody supports investigations into vesicular trafficking and cell signaling regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
