| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STAMBP recombinant protein (Position: K187-R424) was used as the immunogen for the STAMBP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STAMBP Antibody / STAM-binding protein is a anti-STAMBP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: STAMBP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Structurally, STAMBP is a 424-amino-acid cytoplasmic protein of approximately 49 kilodaltons containing a JAMM motif responsible for zinc ion coordination and catalytic activity, as well as SH3-binding regions that enable interaction with signal transducing adaptor molecules (STAM1 and STAM2). It localizes to early endosomes and participates in the endosomal sorting complex required for transport (ESCRT) machinery, modulating trafficking of growth factor receptors such as EGFR and cytokine receptors after ligand binding.
The STAMBP antibody is widely used in cell signaling, membrane trafficking, and neurodevelopmental research to study ubiquitin processing, receptor regulation, and lysosomal degradation pathways. Western blot analysis detects a 49 kilodalton band corresponding to STAMBP, while immunofluorescence reveals punctate endosomal staining. This antibody enables precise detection of endosomal deubiquitinating enzyme activity and ESCRT complex assembly.
Functionally, STAMBP removes Lys63-linked ubiquitin chains from endosomal cargo proteins, facilitating recycling or degradation decisions within the endolysosomal pathway. Its activity prevents overactivation of growth factor signaling and supports normal receptor turnover. Mutations in STAMBP cause microcephaly-capillary malformation syndrome (MIC-CAP), a rare congenital disorder characterized by neurodevelopmental delay, seizures, and vascular malformations resulting from defective ubiquitin recycling. Altered STAMBP expression also contributes to tumor progression and immune dysregulation through prolonged receptor signaling. The STAMBP antibody provides a powerful tool for characterizing ubiquitin-dependent trafficking, growth factor regulation, and endosomal sorting mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
