| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PCTP-L/STARD10 recombinant protein (Position: M1-E273) was used as the immunogen for the STARD10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STARD10 Antibody / StAR-related lipid transfer protein 10 / PCTP-L is a anti-STARD10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: STARD10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, ICC, WB
Biological background
Functionally, STARD10 antibody identifies a 329-amino-acid protein that binds phosphatidylcholine, phosphatidylethanolamine, and other phospholipids through its conserved steroidogenic acute regulatory-related lipid transfer (START) domain. STARD10 facilitates the exchange and transport of phospholipids between membranes, ensuring proper lipid composition of the Golgi apparatus, plasma membrane, and endoplasmic reticulum. It plays important roles in membrane biogenesis, vesicle formation, and signal transduction.
The STARD10 gene is located on chromosome 11q13.1 and is expressed in multiple tissues, including pancreas, liver, and brain. In pancreatic beta cells, STARD10 modulates insulin secretion by regulating lipid signaling and granule membrane dynamics. Genetic studies have linked STARD10 variants to type 2 diabetes susceptibility, indicating its role in glucose metabolism and endocrine function. Its expression is also responsive to cellular lipid levels and nutrient signaling pathways, suggesting involvement in metabolic adaptation.
In lipid metabolism, STARD10 contributes to maintaining phospholipid asymmetry and membrane curvature. It interacts with small GTPases and cytoskeletal components to regulate vesicle trafficking. Dysregulation of STARD10 affects lipid homeostasis and has been associated with metabolic disorders and cancer. Elevated STARD10 expression has been observed in breast and pancreatic cancers, where it may influence membrane signaling and cell proliferation.
STARD10 antibody is widely used in cell biology, metabolism, and endocrinology research. It is suitable for immunoblotting, immunofluorescence, and lipid transport assays to study protein localization and lipid-binding activity. Detection of STARD10 aids in exploring lipid trafficking pathways and their impact on cellular signaling. In disease models, STARD10 antibody supports studies on insulin secretion, metabolic regulation, and tumor lipid metabolism.
Structurally, STARD10 consists of a single START domain forming a hydrophobic binding pocket that accommodates phospholipid molecules. Post-translational modifications such as phosphorylation and acetylation regulate its lipid affinity and subcellular localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
