| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STAT2 recombinant protein (Position: D290-D843) was used as the immunogen for the STAT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STAT2 Antibody / Signal transducer and activator of transcription 2 is a anti-STAT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: STAT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Upon cytokine stimulation, STAT2 undergoes phosphorylation on tyrosine 690 by Janus kinases (JAK1 and TYK2), dimerizes with STAT1, and associates with IRF9. This trimeric complex translocates to the nucleus, where it binds interferon-stimulated response elements (ISREs) in the promoters of interferon-stimulated genes (ISGs), initiating transcription of antiviral effectors such as OAS1, MX1, and ISG15. These gene products suppress viral replication and enhance immune defense mechanisms.
The STAT2 antibody is widely used in immunology, virology, and cell signaling research to examine interferon pathways, innate immune activation, and transcriptional regulation. Western blot analysis detects a 113 kilodalton band corresponding to STAT2, while immunofluorescence reveals both cytoplasmic and nuclear staining following interferon treatment. This antibody enables detailed analysis of IFN-mediated signal transduction and transcriptional regulation in various immune and epithelial cell types.
STAT2 plays a critical role not only in antiviral immunity but also in inflammation, cancer immunosurveillance, and immune tolerance. Deficiency or mutation of STAT2 causes severe susceptibility to viral infections due to impaired interferon signaling, while persistent activation of the JAK-STAT pathway is associated with tumor progression and chronic inflammation. The STAT2 antibody supports exploration of these processes and evaluation of therapeutic modulation of interferon pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
