| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed) |
| Shipping | |
| Species |
Background
STAT3 (signal transducer and activator of transcription 3) is a latent cytoplasmic transcription factor and a central effector of the JAK/STAT signaling pathway. In response to cytokines such as IL-6 and growth factors, receptor-associated Janus kinases phosphorylate STAT3, which then dimerizes, translocates to the nucleus, and binds specific response elements to drive transcription. STAT3 target genes regulate cell proliferation, survival, angiogenesis, and immune responses. Persistent STAT3 activation is a common feature of many cancers and inflammatory conditions, where it promotes tumor growth and immune evasion. Because of this, STAT3 is an extensively studied node in cytokine signaling and oncology research.
Product Description & Applications
The STAT3 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT3 activity in human or mouse cells. Tandem consensus STAT3 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, Renilla luciferase, or SEAP), enabling quantitative monitoring of JAK/STAT pathway activation in transduced cells. Constitutively expressed fluorescent reporters and antibiotic selection markers (puromycin or blasticidin) support tracking of transduced cells and establishment of stable reporter cell lines. The system is used to study cytokine signaling, screen pathway modulators, and characterize STAT3 activity in cancer and inflammation research. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the STAT3 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed)) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
