| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STAT5A/B recombinant protein (Position: K359-R790) was used as the immunogen for the STAT5A/B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STAT5A/B Antibody / Signal transducer and activator of transcription 5 is a anti-STAT5A/B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: STAT5A/B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Upon activation by cytokines such as prolactin, interleukin-2, and growth hormone, STAT5A/B are phosphorylated on a conserved tyrosine residue (Y694 in STAT5A, Y699 in STAT5B), leading to dimerization and nuclear translocation. In the nucleus, they bind specific DNA motifs (GAS elements) to induce transcription of target genes including BCL2L1, CISH, SOCS2, and CCND1. These targets control cell survival, feedback inhibition, and differentiation pathways across multiple systems. STAT5A plays key roles in mammary gland development and lactation, while STAT5B predominantly regulates growth hormone signaling and immune function.
The STAT5A/B antibody is widely used in signal transduction, oncology, and immunology research to detect both STAT5 isoforms in total or phosphorylated forms. Western blot analysis identifies bands near 90 kilodaltons corresponding to STAT5A and STAT5B, while immunofluorescence reveals nuclear accumulation following cytokine stimulation. This antibody supports studies of JAK/STAT pathway activation, cytokine signaling, and transcriptional regulation in various tissues.
Dysregulation of STAT5 signaling is implicated in leukemias, lymphomas, and solid tumors, where constitutive activation promotes oncogenic transformation and resistance to apoptosis. Aberrant STAT5 activity also contributes to autoimmune and inflammatory diseases. The STAT5A/B antibody enables researchers to evaluate STAT pathway activation in both physiological and pathological contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
