| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted.
Detection principle as shown in the figure:
Figure 1: Schematic diagram of the firefly luciferase detection principle
YEASEN Biotech's Steady-One Step Luciferase Reporter Gene Detection Kit is characterized by its ease of operation and a long half-life of over 5 hours, with minimal luminescence value changes in the first 2 hours. It is particularly suitable for ultra-high throughput or 384-well plate detection. There is no need to wash or collect cells before the experiment; the reagent can be used directly, with cell lysis and detection completed in one step, eliminating the need for mixing reagents, thus saving experimental time. This kit achieves a relative balance between signal strength and stability, maintaining a relatively high signal strength while keeping the signal intensity relatively stable over a certain period, ensuring that the experimental results are accurate and reliable. The half-life typically reaches over 1 hour. The product has a wide linear range for sample determination. In a 96-well plate, it can exhibit a good linear relationship within a range of 200,000 cells.
Features
Long signal half-life: The signal half-life is 5 hour, meeting the needs of high-throughput experiments.
Strong fluorescence signal: The signal value can reach 7 orders of magnitude, with a large detection window.
Convenient operation: One-step procedure, avoiding complicated experimental processes.
Applications
Signaling Pathway Research
Candidate Drug Screening
T Cell Killing Assay
Specifications
|
Form |
liquid |
|
Luciferase |
Firefly |
|
Signal Half-life |
5 h |
|
Number of Product Components |
1 |
|
Workflow |
One Step |
Components
|
Components No. |
Name |
11413ES60 |
11413ES80 |
11413ES81 |
|
11413 |
Steady -One Step Luciferase Assay Kit |
10 mL |
10×10 mL |
100 mL |
Shipping and Storage
Steady -One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years.
Figures
Figure1. YEASEN Steady -One Step has better stability and higher signal values compared to its competitors..
Documents:
Safety Data Sheet
Manuals
This kit is compatible with the vast majority of mammalian cell lines and primary cells, including HEK293, HeLa, Jurkat, CHO, and NIH/3T3. The included cell lysis buffer achieves complete lysis of both adherent and suspension cultures. Yeast or bacterial cells are not recommended without protocol adaptation.
A luminometer or microplate reader with a luminescence detection mode is required. Standard fluorescence readers with excitation sources are not suitable. Compatible instruments include the Promega GloMax, BioTek Synergy, PerkinElmer EnVision, or equivalent luminometers. Ensure any injection system is primed before measurement to minimize signal delay.
Luciferase substrates are light-sensitive and mildly temperature-sensitive. Handle under subdued lighting. After preparing working dilutions, use within the timeframe specified in the datasheet (typically 30–60 minutes). Store concentrated substrate stocks at –20°C in amber or foil-wrapped tubes. Do not subject substrates to repeated freeze-thaw cycles.
The linear detection range exceeds 8 orders of magnitude of enzyme concentration, making this kit suitable for detecting both weak promoter activity and highly expressed reporter constructs without signal saturation. This wide dynamic range is particularly valuable for dose-response and titration experiments.
No. This kit is formulated for in vitro cell-based reporter gene assays using cell lysates. For in vivo bioluminescence imaging, use a D-luciferin or coelenterazine formulation validated for in vivo administration. This product is for Research Use Only (RUO) and is not intended for clinical, diagnostic, or in vivo imaging applications.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
