| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
STING (stimulator of interferon genes, encoded by STING1 and also known as TMEM173) is an endoplasmic reticulum adaptor protein central to cytosolic DNA sensing in innate immunity. When the enzyme cGAS detects aberrant cytoplasmic DNA, it produces the second messenger cGAMP, which binds STING and triggers its activation. Activated STING recruits TBK1 to drive IRF3- and NF-κB-dependent transcription, inducing type I interferons and inflammatory cytokines. This cGAS-STING axis is essential for antiviral and antitumor immunity, and its dysregulation contributes to autoinflammatory disease. STING is an actively pursued target for cancer immunotherapy and adjuvant development.
Product Description & Applications
The STING ORF cDNA Lentivirus delivers the full open reading frame of STING for stable overexpression in human and mouse cells. Expression is driven by a CMV promoter, and constructs may include a C-terminal epitope tag, a fluorescent reporter (such as EGFP or mCherry), and a selection marker, with tag, reporter, and marker elements linked by self-cleaving peptide sequences to allow independent protein production.
Supplied as third-generation, VSV-G-pseudotyped lentiviral particles, the product enables efficient transduction of primary, thawed, and other difficult-to-transfect cells and the establishment of stable overexpression cell lines. Applications include gain-of-function studies of cGAS-STING signaling, type I interferon induction, and innate immune responses in cancer and cell biology research.
About This Product
This ORF cDNA lentivirus enables stable overexpression of STING (NCBI Accession: NM_028261, NM_198282) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (EGFP, GFP, mCherry, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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