| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMEM173/STING recombinant protein (Position: L66-K347) was used as the immunogen for the STING1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STING1 Antibody / Stimulator of interferon genes / TMEM173 is a anti-STING1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: STING1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, STING1 antibody identifies a 379-amino-acid endoplasmic reticulum (ER) adaptor protein that senses cyclic dinucleotides (CDNs) generated by the DNA sensor cGAS. Upon binding to cyclic GMP-AMP (cGAMP), STING undergoes conformational changes and translocates from the ER to the Golgi apparatus, where it recruits and activates the kinase TBK1. Activated TBK1 phosphorylates IRF3, triggering transcription of interferon-stimulated genes and cytokines such as IFN-beta, establishing an antiviral state.
The TMEM173 gene is located on chromosome 5q31.2 and encodes a conserved multi-pass transmembrane protein expressed in immune, epithelial, and endothelial cells. STING signaling not only defends against viral infection but also detects self-DNA released during cellular stress, DNA damage, or mitochondrial leakage. While crucial for host defense, persistent STING activation can drive autoinflammation, tissue injury, and autoimmune disease.
STING functions as a master regulator of cytosolic DNA responses, mediating immune recognition of viral, bacterial, and tumor DNA. Gain-of-function mutations in TMEM173 cause STING-associated vasculopathy with onset in infancy (SAVI), a severe autoinflammatory disorder characterized by excessive interferon signaling. Conversely, impaired STING activation increases susceptibility to infection and impedes tumor immune surveillance. In oncology, STING serves as a therapeutic target for cancer immunotherapy, where agonists are used to boost antitumor immunity through interferon induction and dendritic cell activation.
STING antibody is widely used in immunology, oncology, and inflammation research. It is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect endogenous STING protein and study its activation dynamics. This antibody supports investigations into innate immune signaling, interferon pathway regulation, and host-pathogen interactions. In cancer studies, STING detection helps evaluate immune activation following treatment with STING agonists or DNA-damaging agents.
Structurally, STING forms a homodimer with a cytosolic ligand-binding domain that interacts with cGAMP and CDNs. The protein contains four transmembrane helices anchoring it to the ER membrane and a C-terminal tail essential for TBK1 and IRF3 recruitment.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
