| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STK16 recombinant protein (Position: E109-L268) was used as the immunogen for the STK16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STK16 Antibody / Serine/threonine-protein kinase 16 is a anti-STK16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus, Cell membrane.
Key elements and design rationale
- Target: STK16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, WB, IHC, FACS
Biological background
Functionally, STK16 antibody identifies a 305-amino-acid protein with a typical serine/threonine kinase domain and a C-terminal myristoylation site that anchors it to membranes. STK16 phosphorylates various substrates involved in exocytosis, including components of the SNARE complex. It is essential for maintaining Golgi structure, regulating secretion, and coordinating protein sorting within the secretory pathway.
The STK16 gene is located on chromosome 2q31.1 and is ubiquitously expressed, with enrichment in liver, pancreas, and secretory epithelial tissues. STK16�s kinase activity is regulated by autophosphorylation and membrane association. It also participates in cell cycle progression and polarized transport, suggesting broader roles in organelle biogenesis and signal transduction.
Pathologically, aberrant STK16 function has been implicated in tumorigenesis, metabolic disease, and Golgi stress. Overexpression may enhance secretion and proliferation, while inhibition disrupts vesicular trafficking and protein glycosylation. Research using STK16 antibody contributes to understanding kinase-mediated control of membrane traffic and its implications in disease and cellular physiology.
STK16 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to visualize Golgi-associated kinases and phosphorylation-dependent trafficking.
Structurally, STK16 belongs to the NAK (Numb-associated kinase) family and features an N-lobe typical of protein kinases, with regulatory elements that stabilize its constitutive activity. This antibody supports detailed characterization of STK16's role in kinase signaling and Golgi organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
