| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STK17A recombinant protein (Position: Q38-Q383) was used as the immunogen for the STK17A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STK17A Antibody / Serine/threonine-protein kinase 17A is a anti-STK17A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: STK17A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
STK17A is encoded by the STK17A gene on human chromosome 7p13. The protein is approximately 42 kilodaltons and consists of an N-terminal catalytic kinase domain and a C-terminal regulatory tail rich in serine residues. STK17A localizes mainly to the nucleus and cytoplasm, where it phosphorylates substrates involved in apoptosis signaling and actin remodeling.
The STK17A antibody detects a 47-53 kilodalton band by western blot and demonstrates nuclear enrichment under apoptotic conditions. STK17A mediates cell death through phosphorylation of transcription factors such as FOXO3 and histone H2B, promoting DNA fragmentation and chromatin condensation. Its expression is upregulated by p53 following DNA damage, placing it downstream of tumor-suppressive signaling pathways.
STK17A also influences cytoskeletal dynamics by modulating RhoA activity and actin filament organization, linking apoptosis regulation to cellular morphology. In neurons, STK17A activation contributes to axon retraction and degeneration during stress. Conversely, suppression of STK17A enhances survival and regenerative capacity in damaged tissues.
Dysregulated STK17A signaling is implicated in multiple diseases. Overexpression promotes apoptosis and tissue atrophy, while reduced activity correlates with tumor survival and resistance to therapy. Pharmacologic targeting of STK17A could restore controlled cell death in cancer or prevent neurodegeneration in stress-related disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
