| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STK31 recombinant protein (Position: H29-K993) was used as the immunogen for the STK31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STK31 Antibody / Serine/threonine-protein kinase 31 is a anti-STK31 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: STK31
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, WB, IHC, FACS
Biological background
Functionally, STK31 antibody identifies an 888-amino-acid cytoplasmic and nuclear protein containing a serine/threonine kinase domain and a Tudor domain that recognizes methylated arginine and lysine residues. STK31 acts as a scaffold linking chromatin dynamics to signaling pathways that govern cell division. In germ cells, it localizes to the chromatoid body, a specialized cytoplasmic structure involved in RNA processing and regulation during spermatogenesis. Its activity supports meiotic progression, chromatin condensation, and genomic stability.
The STK31 gene is located on chromosome 7p15.3 and is expressed predominantly in testis, with lower levels detected in certain somatic tissues and tumors. Its kinase activity regulates phosphorylation events critical for spindle assembly and chromosome alignment. Dysregulation of STK31 expression has been observed in various cancers, including colorectal, gastric, and breast carcinoma, where it may promote cell proliferation and migration through modulation of cytoskeletal organization and mitotic checkpoint control.
In cancer biology, STK31 is considered a cancer-testis antigen, reflecting its restricted normal expression but reactivation in tumors. Elevated expression enhances proliferation and resistance to apoptosis, suggesting potential as a therapeutic target or biomarker. STK31 overexpression also correlates with chromosomal instability and altered epigenetic landscapes in tumor cells.
STK31 antibody is widely used in cell cycle, germ cell biology, and oncology research. It is suitable for immunoblotting, immunofluorescence, and kinase activity assays to detect STK31 expression and subcellular localization. In reproductive biology, it supports studies of meiotic regulation, RNA granule organization, and germ cell maturation. In oncology, detection of STK31 contributes to understanding its role in tumorigenesis and potential as a prognostic marker.
Structurally, STK31 comprises a conserved catalytic kinase domain, a Tudor domain for chromatin interactions, and regulatory sequences that control subcellular targeting. Its kinase activity may be regulated by phosphorylation and binding to scaffold proteins within the mitotic apparatus.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
