| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STK32C recombinant protein (Position: Q42-M475) was used as the immunogen for the STK32C antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STK32C Antibody / Serine/threonine-protein kinase 32C is a anti-STK32C Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: STK32C
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
STK32C is encoded by the STK32C gene located on human chromosome 5q35.1. The protein is approximately 56 kilodaltons and contains a conserved serine/threonine kinase catalytic domain with typical subdomains I-XI, including an ATP-binding site and an activation loop. Expression profiling shows broad distribution across tissues, with enriched levels in brain, heart, and skeletal muscle, suggesting roles in excitable tissue function and mitochondrial homeostasis.
STK32C phosphorylates substrates involved in metabolic regulation and cytoskeletal dynamics, though its full substrate repertoire remains under study. In neuronal systems, STK32C expression increases during differentiation, implying a developmental regulatory function. Comparative analysis with STK32A and STK32B reveals overlapping but distinct substrate preferences and signaling responses, highlighting potential functional diversification among this kinase subfamily.
Recent phosphoproteomic analyses indicate that STK32C may participate in stress-activated signaling and mitochondrial fission control. Inhibition or silencing of STK32C disrupts mitochondrial morphology and reduces oxidative phosphorylation efficiency. This kinase may integrate metabolic status with energy homeostasis by phosphorylating mitochondrial or cytosolic target proteins. Additionally, preliminary data suggest a role in autophagic flux modulation and endoplasmic reticulum-mitochondria communication.
In cancer biology, STK32C has been implicated in cellular proliferation and invasion through phosphorylation of cytoskeletal effectors. Elevated expression correlates with poor prognosis in certain neuroblastoma and glioblastoma datasets, suggesting possible oncogenic behavior in nervous system tumors. In contrast, other studies report STK32C downregulation in degenerative neural conditions, implying context-dependent effects. The enzyme's unique sequence features, including an elongated C-terminal tail and regulatory motifs, suggest fine-tuned signaling modulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
