| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human STOML3 recombinant protein (Position: D2-A291) was used as the immunogen for the STOML3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STOML3 Antibody / Stomatin-like protein 3 is a anti-STOML3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: STOML3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
STOML3 plays a crucial role in peripheral mechanosensation by enhancing the responsiveness of mechanosensitive ion channels to mechanical stimuli. It forms oligomeric complexes that influence channel gating and localization within mechanosensory end-organs. In dorsal root ganglion neurons, STOML3 interacts with PIEZO2 to amplify mechanosensitive currents required for touch and proprioception. Genetic deletion of STOML3 in mice leads to significant deficits in mechanical sensitivity without affecting nociception, demonstrating its specificity for tactile function.
The STOML3 antibody is widely used in neurobiology and sensory physiology research to detect Stomatin-like protein 3 and study its interactions with mechanosensory ion channels. Western blot analysis identifies a 32 kilodalton band corresponding to STOML3, while immunofluorescence shows punctate cytoplasmic staining in sensory neurons and mechanosensory tissues. The antibody allows visualization of STOML3 localization within neuronal projections and sensory end-organs, supporting investigations into the molecular basis of touch sensation.
Beyond sensory neurons, STOML3 expression has been reported in lung and kidney tissues, suggesting broader roles in mechanotransduction and membrane curvature regulation. It may also participate in lipid raft organization and vesicular trafficking. The STOML3 antibody enables researchers to explore these processes and provides a key reagent for examining the molecular architecture of mechanosensitive signaling complexes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
