| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STRBP recombinant protein (Position: K110-R617) was used as the immunogen for the STRBP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STRBP Antibody / Spermatid perinuclear RNA-binding protein is a anti-STRBP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: STRBP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Spermatid perinuclear RNA-binding protein contains multiple double-stranded RNA-binding motifs (dsRBDs) and a leucine zipper domain that mediate RNA recognition and dimerization. It localizes to the cytoplasm and perinuclear region, where it associates with RNA granules and microtubules. The STRBP antibody supports detection of this localization, enabling studies of its role in mRNA transport and translational control. STRBP is particularly enriched in developing spermatids, where it participates in the storage and regulation of mRNAs required for sperm maturation.
Beyond reproductive function, Spermatid perinuclear RNA-binding protein is expressed in multiple tissues, including brain and heart, where it modulates RNA metabolism and cytoskeletal integrity. It binds to microtubule-associated complexes to stabilize RNA localization during cellular polarization. The STRBP antibody enables visualization of these interactions, providing insight into how STRBP integrates RNA processing with cytoskeletal rearrangement in differentiating cells.
STRBP also plays a role in stress granule formation during cellular stress, interacting with proteins such as Staufen and DDX3X to regulate mRNA triage and translation repression. The STRBP antibody supports studies of these dynamic processes, helping to elucidate the molecular mechanisms underlying mRNA storage and recovery following stress exposure. Dysregulation of STRBP has been linked to infertility, neurodevelopmental disorders, and altered stress responses.
The STRBP antibody is validated for western blotting, immunofluorescence, and immunohistochemistry, producing strong cytoplasmic and perinuclear staining consistent with its RNA granule localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
