| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Syntaxin 10/STX10 recombinant protein (Position: K77-K218) was used as the immunogen for the STX10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STX10 Antibody / Syntaxin 10 is a anti-STX10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Golgi apparatus.
Key elements and design rationale
- Target: STX10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
STX10 is essential for maintaining endosomal organization and cargo specificity, particularly in the recycling of mannose 6-phosphate receptors (MPRs) that deliver lysosomal hydrolases. It contributes to the tethering and fusion of vesicles returning from late endosomes to the TGN, a process required for proper lysosome formation and enzyme sorting. Loss of STX10 function disrupts endosomal integrity and leads to defective lysosomal enzyme trafficking, underscoring its role in vesicle-mediated transport.
The STX10 antibody is widely used in cell biology, membrane trafficking, and neurobiology research to investigate SNARE-mediated vesicular transport. Western blot analysis identifies a 30 kilodalton band corresponding to Syntaxin-10, while immunofluorescence reveals punctate perinuclear staining that colocalizes with Golgi and endosomal markers such as TGN46 and Rab9. This antibody supports detailed analysis of intracellular trafficking, organelle organization, and vesicle fusion mechanisms.
Beyond its role in vesicle trafficking, STX10 participates in endosome-to-Golgi transport pathways that maintain protein homeostasis and receptor recycling. It also contributes to specialized trafficking functions in neurons, immune cells, and epithelial tissues. Dysregulation of SNARE complex components, including STX10, has been implicated in neurodegenerative diseases and metabolic disorders where vesicle trafficking pathways are compromised. The STX10 antibody enables researchers to explore these processes and assess Syntaxin-10 function in both normal and pathological conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
