| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Syntaxin 4/STX4 recombinant protein (Position: M1-I278) was used as the immunogen for the STX4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STX4 Antibody / Syntaxin 4 is a anti-STX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, cytoplasm.
Key elements and design rationale
- Target: STX4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Structurally, Syntaxin 4 is a 273-amino-acid membrane protein of approximately 33 kilodaltons containing a C-terminal transmembrane anchor, a SNARE motif required for complex formation, and an N-terminal regulatory domain. It localizes to the plasma membrane and cytoplasmic vesicles in diverse tissues including adipose, neuronal, and epithelial cells. Upon stimulation, Syntaxin 4 assembles into heterotrimeric SNARE complexes that catalyze the fusion of transport vesicles with target membranes.
The STX4 antibody is widely used in cell biology, metabolism, and neurophysiology research to study vesicular trafficking, membrane fusion, and regulated exocytosis. Western blot analysis detects a 33 kilodalton band corresponding to Syntaxin 4, while immunofluorescence reveals peripheral and punctate membrane staining. This antibody provides a reliable reagent for analyzing vesicle dynamics and fusion machinery in secretory systems.
Functionally, Syntaxin 4 is essential for insulin-dependent GLUT4 trafficking in adipocytes and muscle cells, linking it to glucose uptake and metabolic homeostasis. It also contributes to immune cell secretion of cytokines and cytotoxic granules, as well as neurotransmitter release in the nervous system. Disruption of STX4 impairs exocytic processes and contributes to metabolic disorders such as type 2 diabetes. The STX4 antibody supports studies exploring SNARE-mediated exocytosis, vesicle targeting, and signal-dependent membrane fusion.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
