| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STX5 recombinant protein (Position: M1-Q327) was used as the immunogen for the STX5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STX5 Antibody / Syntaxin 5 is a anti-STX5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi).
Key elements and design rationale
- Target: STX5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Syntaxin-5 is encoded by the STX5 gene located on human chromosome 11q12.2. The protein exists as two isoforms generated by alternative translation initiation, a long (42 kilodalton) and short (35 kilodalton) form, both containing an N-terminal coiled-coil region and a C-terminal transmembrane domain. These isoforms localize to different Golgi subcompartments and coordinate SNARE complex formation with partners such as Bet1, Sec22b, and GosR2 to drive vesicle docking and fusion.
The STX5 antibody detects a characteristic double band in western blot corresponding to both isoforms. Immunofluorescence typically shows strong perinuclear staining consistent with Golgi localization. Functionally, Syntaxin-5 plays a pivotal role in maintaining Golgi structure and retrograde transport from the Golgi to ER. Disruption of STX5 function leads to ER stress, defective glycosylation, and impaired secretion of transmembrane and secretory proteins.
Syntaxin-5 also participates in autophagy and lipid metabolism by regulating membrane flow between organelles. Mutations in STX5 cause congenital disorders of glycosylation and multisystemic developmental delay. The protein further contributes to viral replication processes, as certain viruses exploit STX5-dependent pathways to reorganize host membranes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
