| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids D57-E481 from the human protein were used as the immunogen for the STXBP4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STXBP4 Antibody / Syntaxin-binding protein 4 is a anti-STXBP4 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: STXBP4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P, FACS, ELISA
Biological background
STXBP4 is particularly significant in processes related to glucose metabolism. It has been shown to regulate the trafficking of the glucose transporter GLUT4 to the plasma membrane in muscle and adipose tissue. This regulation is critical for insulin-stimulated glucose uptake, linking STXBP4 directly to metabolic homeostasis. Alterations in its expression or activity have been connected to insulin resistance and type 2 diabetes, making it an important subject of investigation in metabolic disease research. Beyond glucose regulation, STXBP4 participates in broader endocytic and exocytic pathways that influence receptor signaling and cellular communication.
Studies suggest that STXBP4 may also contribute to cancer biology. Dysregulated expression has been observed in certain tumor types, where altered vesicle trafficking can affect growth factor signaling and proliferation. Research is ongoing to determine whether STXBP4 serves as a biomarker for cancer progression or a potential therapeutic target. Its involvement in vesicle-mediated signaling suggests that it may influence multiple pathways central to tumor growth and survival.
At the molecular level, STXBP4 is characterized by conserved domains that enable binding to syntaxins and interaction with SNARE complexes. This interaction stabilizes vesicle docking and coordinates the timing of fusion events. Experimental models demonstrate that modulation of STXBP4 can alter the efficiency of GLUT4 vesicle fusion, highlighting its central role in metabolic regulation. Antibody-based approaches are frequently used to assess protein levels, localization, and interactions within these pathways.
The STXBP4 antibody is widely applied in techniques such as western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. These applications allow scientists to investigate expression across tissues, examine subcellular distribution, and evaluate changes in disease states. When used alongside functional assays, STXBP4 detection provides valuable insight into vesicle trafficking and its consequences for metabolism and signaling. For investigators examining insulin action, metabolic disorders, or vesicular transport, the STXBP4 antibody is a reliable and versatile research tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
