| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human STYK1 recombinant protein (Position: M1-Y404) was used as the immunogen for the STYK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
STYK1 Antibody / Serine/threonine/tyrosine kinase 1 is a anti-STYK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: STYK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Structurally, STYK1 is composed of a short extracellular domain, a single transmembrane helix, and a cytoplasmic kinase domain that mediates signal transduction. Although structurally similar to receptor tyrosine kinases, it lacks a classical ligand-binding domain and likely operates through intracellular signaling or interaction with other membrane proteins. STYK1 activates downstream effectors, including the MAPK/ERK and PI3K/AKT pathways, promoting cell survival and motility.
The STYK1 antibody is widely used in cancer biology, kinase signaling, and molecular pathology research to investigate its oncogenic potential and functional roles in signal integration. Western blot analysis detects a 48 kilodalton band corresponding to STYK1, while immunohistochemistry shows strong membrane and cytoplasmic staining in epithelial tissues and cancer cells. This antibody provides a sensitive tool for evaluating kinase expression patterns and activation states in tumor and normal tissues.
Overexpression of STYK1 has been documented in lung, breast, prostate, and ovarian cancers, where it enhances tumor cell proliferation, metastasis, and resistance to apoptosis. It may function as a pseudokinase or a signaling scaffold that amplifies receptor-mediated responses. In normal physiology, STYK1 contributes to embryonic development and tissue regeneration by regulating kinase cross-talk. The STYK1 antibody enables researchers to dissect these mechanisms and explore its potential as a therapeutic target in oncology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
